... Matsuda H: Primary germcelltumors in the mediastinum: a 50-year experience at a single Japanese institution Cancer 2003, 97(2):367-376 Droz JP, Horwich A: Extragonadal germcelltumors Comprehensive ... patient’s pituitary showing polymorphic cells with granular cytoplasm Discussion This case study is unusual because our patient had both SLE and pituitary germcell tumor It is also unusual because ... Rheum 2003, 48:2100 doi:10.1186/1752-1947-4-97 Cite this article as: Shahrir et al.: Extragonadal germcell tumor presenting in a woman with systemic lupus erythematosus: a case report Journal of...
... Features Assay type Cell types Cells enter or cross cellulose membrane Cells move toward and enter capillary filled with attractant Cells avoid capillary filled with control buffer Cells migrate in ... and direction changes of individual cells (together referred to as “tracking” assays) as well as determinations of cell orientation, cellcell interactions, cell morphology, flagellar wave patterns, ... reservoirs; cells adhere to cover glass during migration Cells migrate between cover glass and slide; cells and chemoattractant released from wells Cells migrate across a circular bridge separating cell...
... receptors in giant celltumors (GCTs) of bone Surgical specimens were fixed in formalin and serially sectioned (a) VEGF was expressed in stromal cells (arrows), monocyte-like cells (arrowheads) ... VEGF was clearly expressed in stromal cells, monocyte-like cells and MNCs in GCTs CD68, an intracellular glycoprotein, was expressed in monocyte lineage cells, including OPCs and OCs [20] Therefore, ... washed with PBS Then, the cells were stimulated with VEGF and GCT-CM with or without ZD4190 for 24 h Cellular proliferation was assessed by the Celltiter-Glo Luminescent Cell Viability Assay Results...
... receptors in giant celltumors (GCTs) of bone Surgical specimens were fixed in formalin and serially sectioned (a) VEGF was expressed in stromal cells (arrows), monocyte-like cells (arrowheads) ... VEGF was clearly expressed in stromal cells, monocyte-like cells and MNCs in GCTs CD68, an intracellular glycoprotein, was expressed in monocyte lineage cells, including OPCs and OCs [20] Therefore, ... washed with PBS Then, the cells were stimulated with VEGF and GCT-CM with or without ZD4190 for 24 h Cellular proliferation was assessed by the Celltiter-Glo Luminescent Cell Viability Assay Results...
... Human cell lines used were HeLa cervical carcinoma cells, HepG2 hepatoma cells, K562 and YN-1 erythroleukemia cells, Jurkat T-lymphocyte cells, KG1 myeloid cells, H146 small cell lung cancer cells, ... and HMV-II melanoma cells H146 small cell lung cancer cells were obtained from ATCC (HTB-173) and cultured in RPMI-1640 medium HMV-II melanoma cells were obtained from Riken Cell Bank and cultured ... hemoglobin production in YN-1 and K562 cells [33,34] In fact, the population of hemoglobin-positive cells was about 4.4% in YN-1 cells and 4.9% in K562 cells under basal conditions [19] Otherwise,...
... previously described [21] However for easier handling of the cells, treatment was performed on HeLa cells instead of CHO K1 cells Cells were then incubated with of lgámL)1 Tat±GFP fusion protein ... same cell lines, namely wild-type (wt) CHO-K1 cells and A-745 mutant cells which are completely defective in HS sulfate expression [21] As a positive control, uptake of the Tat peptide in HeLa cells ... same extent in HeLa cells, in wt-CHO cells and in the A-745 (defective in HS proteoglycan) mutant cell line Moreover internalization appeared to be homogeneous in the whole cell population as...
... specifically expressed in germ cells of both male and female oysters, and we hypothesized that Oyvlg had a role in germcell formation [17] However, the function of Oyvlg in germline development had ... reproductive deficiency The premeiotic male germ cells ceased their differentiation before the pachytene In vivo injection of oyvl-dsRNA provokes abnormal germcell development One month postinjection, ... defects in germcell development affecting all of the gonadic area, in both females and males Upon histological examination of gonads injected with 20 lg of dsRNA, there were fewer germ cells, and...
... vitro cell- free methods for protein synthesis with extracts from prokaryotic [1] or eukaryotic [2] cells Wheat germ cell- free eukaryotic protein production offer an alternative to the E coli cell- based ... wheat germ cell- free method comparable to that of the E coli cell- based approach for NMR structure determinations of eukaryotic proteins One of the main advantages of the automated wheat germ cell- free ... normal multinuclear NMR spectra Cell- free protein production protocols are streamlined compared to cell- based protocols, in that they not require cell harvesting or cell lysis Protein purification...
... testicular Leydig cells in vitro [11] 874 Germ cells constitute the major inner seminiferous tubular cell mass and the role of H2O2 as a signalling molecule in apoptotic induction among germ cells is ... penicillin The cell viability of the final crude population that contained mostly maturing germ cells was in excess of 95% H2O2 treatment The isolated testicular germ cells (5 · 106 cellsÆmL)1 per ... Involvement of Bcl-2 family proteins in germcell apoptosis during testicular development in the rat and pro-survival effect of stem cell factor on germ cells in vitro Mol Cell Endocrinol 165, 115–129 25...
... Testicular GermCellTumors 75 Delphine Carouge and Joseph H Nadeau Chapter Epigenetic Modifications in Testicular GermCellTumors 107 Christopher J Payne Chapter Claudins and GermCellTumors Ylermi ... options for the most common type of germcelltumors of the testes, non-seminomatous germcelltumors In Chapter 3, Takamitsu Fujimaki reviews intracranial germcell tumors, which affect mostly children, ... presentation, current treatment modalities and the biology and genetics of germcelltumors Most authors have focused on testicular germcelltumors which are the most common cancers in young adult males...
... protein N-myristoylation in plant cells in more detail, we established a cell- free protein N-myristoylation system as an application of an advanced wheat germ cell- free translation system that ... Myr–GFP synthesized in the wheat germ cell- free translation system Effect of the combination of amino acids at positions and on protein N-myristoylation in the wheat germ cell- free translation system ... and of the consensus motif in plant target proteins with the use of a Cell- free N-myristoylation of plant proteins wheat germ cell- free translation system We identified several new N-myristoylated...
... midgestation of putative germ cell- derived clones, we hypothesize that either gonadal cells other than germ cells may have been picked instead of true germ cells or that a subset of germ cells may not have ... developmental potential of germcell nuclei after transfer into oocytes remains controversial To date, gonadal cells bearing morphological resemblance to germ cells have been used or germ cells have been ... donor cells, either ES, EC, or germ cells could be used In this study, germ cells are used as they match cumulus cells for direct recovery from the body and absence of in vitro culture Gonadal cells,...
... of renal tubules representing the origin of clear cell renal cell carcinoma (RCC) and in the regulation of the cell cycle of parietal epithelial cells of the Bowman's capsule [10,11] Since UCHL1 ... degradation pathway plays an important role in distinct cellular processes such as cell proliferation, cell cycle, apoptosis and intracellular signalling [8], which are often disturbed in cancers ... the expression of UCHL1, RCC cell lines were treated for days with 1, and 10 μM DAC (Sigma-Aldrich GmbH, Taufkirchen, Germany) Subsequently untreated and DAC treated cells were harvested, lysed...
... on fusion of TF228.1.16 cells with SupT1 cells Effect of3 Effect of IM28 on fusion of TF228.1.16 cells with SupT1 cells TF228.1.16 cells were mixed with SupT11 cells (1:1 cell ratio) and transferred ... TF228.1.16 cells were mixed with 293/CD4+ cells (1:1 cell ratio) and transferred to a 24-well plate (105 cells per well in 200 µl of culture medium) TF228.1.16 cells and 293/CD4+ cells were incubated ... HIV-1-infected cells to naive cells We found that IM28 was able to inhibit cell- cell fusion in an in vitro system We showed that IM28 significantly blocks HIV-1 glycoprotein-mediated cell- cell fusion...
... on fusion of TF228.1.16 cells with SupT1 cells Effect of3 Effect of IM28 on fusion of TF228.1.16 cells with SupT1 cells TF228.1.16 cells were mixed with SupT11 cells (1:1 cell ratio) and transferred ... TF228.1.16 cells were mixed with 293/CD4+ cells (1:1 cell ratio) and transferred to a 24-well plate (105 cells per well in 200 µl of culture medium) TF228.1.16 cells and 293/CD4+ cells were incubated ... HIV-1-infected cells to naive cells We found that IM28 was able to inhibit cell- cell fusion in an in vitro system We showed that IM28 significantly blocks HIV-1 glycoprotein-mediated cell- cell fusion...
... Leydig cell Sertoli cell Spermatogenic cell 1+ 2+ 1+ 1+ 2+ 1+ * = no staining, 1+ = <10% positive cells, 2+ = 10 to 50% positive cells, 3+ = >50% positive cells ... and Sertoli celltumors filled the lumens or sheets of the affected seminiferous tubules These cells replaced the normal lining of spermatogenic and Sertoli cells Large and irregular cellular aggregates ... and canine Canine seminomas and Sertoli celltumors testicular tumors In the current seminoma series, inhibin-alpha was observed in the cytoplasm of tumorscell in 45% of the cases Vimentin and...
... 4.0% for renal cell carcinoma, 0.84% to 3.6% for transitional cell carcinoma, 0.4% for germcell tumors, and 0.36% to 0.7% for prostate adenocarcinoma[9] Testicular mixed germcelltumors are common, ... common, comprising roughly 33% of all testicular tumors The reason for such a high incidence of testicular mixed germ- celltumors is because germ cells in the testes are totipotent and can undergo ... testicular tumors reported in the annual of pathological autopsy cases in Japan Hinyokika Kiyo 1983, 29:155-68 12 Bredael JJ, Vugrin D, Whitmore WF Jr: Autopsy findings in 154 patients with germcell tumors...
... giant celltumors occurring outside the extremities in combination with a short review of the literature Patients and Methods Between 2000 and 2006 a total of five patients with giant celltumors ... All tumors were histologically proven before start of the treatment All patients except one with a giant cell tumor in the sphenoid sinus suffered from large tumors in the sacral region Three tumors ... 25 (8%) patients after surgery The appearance of malignant giant celltumors of bone or malignant foci inside benign giant celltumors has been described also in a small number of patients [24,25],...
... established human B -cell tumor lines and fresh patient samples representing the spectrum of B -cell tumors in man Our results demonstrate activity of TW-37 across all B -cell tumors irrespective ... complexity in B -cell tumors Our results presented here, show that small-molecule inhibitors of the Bcl-2 family proteins has a therapeutic role in a wide spectrum of B -cell tumors All four cell lines ... cytotoxic effect on primary lymphoma cells The CLL/SLL cells expressed CD5, CD19, CD20 and faint monotypic SIg The MZL cells were CD5-, CD19+ and CD20+ Mononuclear cells were separated by Ficoll-Hypaque...
... (Figure 1c) The diagnosis was of an International GermCell Cancer Collaborative Group (IGCCCG) poor prognosis metastatic nonseminomatous germcell tumor Four cycles of bleomycin, etoposide and ... and is mainly a result of local spread from the surrounding organs Germcelltumors of the testis are the most common solid tumors in men aged between 20 and 35 years We are not aware of any ... European Consensus Conference on Diagnosis and Treatment of GermCell Cancer: A report of the Second Meeting of the European GermCell cancer Consensus group (EGCCCG): Part Eur Urol 2008, 53:478-496...